Optical Filters for Fluorescence Techniques

The emission of light by a substance that has absorbed any kind of electromagnetic radiation is known as fluorescence. There is a range of analysis techniques that utilize the fluorescence phenomenon, and Delta Optical Thin Film supply a range of optical filters that can be used for these techniques.

This blog post will discuss some of the fluorescence related techniques that our optical filters can help to perform.

Fluorescence Microscopy

Widely used in the biological and medical fields, fluorescence microscopy detects signals that are 1 million times weaker than the light that illuminates the sample.

To be successful, the technique relies on the detection of fluorescence signals that are 1000 times weaker than scattered light, which is 1000 times weaker than the light that illuminates the sample.

This is not simple to achieve and obtaining the correct result relies on the use of high-performance optical filters and dichroic beamsplitters in fluorescence microscopes.

Flow Cytometry

Flow cytometry has a large range of applications, from the diagnosis of health disorders to basic research and clinical trials. Similar to fluorescence microscopy, flow cytometry requires powerful optical filters to obtain the desired results.

This is due to the fact that emission and absorption spectra of most fluorescent dyes are located closely together and often overlap. So, emission and excitation filters are required to have very steep edges in order to effectively separate absorption and emission peaks.

To make the weak fluorescence signal detectable in the strong illumination light, high out-of-band rejection is required.

Fluorescence Spectroscopy

Fluorescence spectroscopy is a technique employed by chemists who want to investigate the structure of unknown compounds. The technique involves the submission of a sample to radiation or light, and the interaction is examined.

An important component in a spectrometer is a wavelength selector, and interference filters are a common type of wavelength selector.

Compared to another type of wavelength selector, the monochromator, interference filters are much smaller and lighter. They also provide technical benefits, including increased potential grasp of energy.

Interference filters are generally composed of several hundred optical layers, deposited on a transparent glass or quartz substrate. The specific performance properties of the filter are determined by the thickness and sequence of the optical layers.

Optical Filters for Fluorescence Techniques from Delta Optical Thin Film

Delta Optical Thin Film was the first to suggest interference filters to companies including Leica and Carl Zeiss in the 1960s. In the 1970s we started to manufacture fluorescence filters in mass volumes and in the early 1990s we were the first company to implement computer controlled deposition into our manufacturing process.

Our unique control and synthesis control software allows us to provide coatings that are made with plasma assisted e-beam evaporation technology. This process results in coatings with a quality equal to that achieved with IBS and similar technologies.

Delta Optical Thin Film are the biggest suppliers of high-performance fluorescence filter sets to the market leaders in the fluorescence microscopy sector. If you require any information about any of our optical filters for fluorescence techniques please contact us.