Fluorescence Microscopy: Understanding Fluorophores

Fluorescence microscopy is a method of observing photo-emissive spectra from samples, and acquiring fluorescent signals that are roughly one million times weaker than the incident or one thousand times weaker than the scattered light. Observing the fluorescence of an analyte requires a sensitive array comprising an incident light source and robust optical filters with exacting […]

Hyperspectral Imaging Applications

Hyperspectral imaging is the process of photographically acquiring data from across the electromagnetic spectrum, with the aim of obtaining spectral information in each pixel of a captured image. This detailed technique uses multiple imaging methodologies to photograph a scene across the spectral range in many contiguous spectral bands. Hyperspectral imaging spectrometers cover wavelengths beyond the […]

What Are Optical Bandpass Filters?

An optical bandpass filter is a spectrally selectively transparent component capable of transmitting select wavelengths of light while blocking others. They are fully customisable to varying center wavelengths and bandwidths, enabling their application in a broad range of practical applications including fluorescence spectroscopy, microscopy, and flow cytometry. Delta Optical Thin Film previously explored the range […]

Using Continuously Variable Filters for Fluorescence Spectroscopy

Fluorescence spectroscopy is the processes of analyzing the emissive, spectral characteristics of a fluorescent compound which has been excited by photons of light. Typically, a sample will fluoresce light with longer wavelengths thus lower energy than that which it absorbed, requiring sensitive equipment with a broad wavelength range and high optical blocking (OD) to accurately […]

Applications for Fluorescence Microscopy

Fluorescence microscopy is a technique used to analyze biological structures in a sample using a white lamp, and either organic or inorganic fluorophores such as dyes to excite a photo-emissive reaction, which is observed using an optical bandpass filter and a dichroic mirror. Various biochemical industries rely on fluorescence microscopy for the performance of molecular […]

What is Laser Raster Microscopy?

Laser raster microscopy, or confocal laser scanning microscopy (CLSM), is an optical analysis technique for fluorescence microscopy. It is a broadly applied image analysis method with applications in biological and medical research, and utilizes a combination of a diffraction-limited laser, beamsplitters, and bandpass filters, to observe the excitation of photo-fluorescence particles, or fluorescent dyes in […]

Optical Filters for Fluorescence Microscopy

Fluorescence microscopy is an imaging technique that is often employed in medical research and biological applications. The technique uses fluorescence and phosphorescence in addition to, or instead of absorption or reflection to study the properties of a substance. Fluorescence microscopy is a popular technique as fluorescence has extremely high sensitivity and specificity, which helps to […]

What is Fluorescence Spectroscopy?

Fluorescence is the phenomenon where a molecule absorbs light within its absorption band and then emits this light at longer wavelengths within its emission band. This phenomenon can be used to identify, quantify, and observe chemical activity, and it is a popular method due to its high levels of sensitivity, simplicity, and specificity. Fluorescence spectroscopy […]

Linear Variable Filter or Continuously Variable Filter?

Over the past six years, Delta Optical Thin Film A/S have been producing and further developing spatially variable filters. These types of filters have spectral characteristics that change spatially and were around for several years before we started to develop our own. Traditionally, when people use the term linear variable filter they mean a filter […]

Delta Optical Thin Film Linear Variable Filter Used in new Laser Attenuator

A new Laser Attenuator has been developed, which utilizes an ultra-hard coated, Laser Optical Multilayer Attenuator (LOMA) filter from Delta Optical Thin Film. The laser attenuator enables computer-controlled attenuation of a monochromatic light source from 1% – 99% within the wavelength region of ~ 550 nm – 1000 nm. It provides the user with safe, […]